Midgut Volvulus Adds a Murine, Neutrophil-Driven Model of Septic Condition to the Experimental Toolbox

Midgut Volvulus Adds a Murine, Neutrophil-Driven Model of Septic Condition to the Experimental Toolbox

Blog Article

Background: Severe infections that culminate in sepsis are associated with high morbidity and mortality.Despite continuous efforts in basis science and clinical research, evidence based-therapy is mostly limited to basic causal and supportive measures.Adjuvant therapies often remain without clear evidence.The objective of this study was to evaluate the septic volvulus ischemia-reperfusion model in comparison to two Tank already established models and the role of neutrophil extacellular traps (NETs) in this model.

Methods: The technique of the murine model of midgut volvulus was optimized and was compared to two established models of murine sepsis, namely cecal ligation and puncture (CLP) and intra-peritoneal (i.p.) injection of lipopolysaccharide (LPS).Results: Midgut volvulus for 15 min caused a comparable mortality (38%) as CLP (55%) and peritoneal LPS injection (25%) at 48 h.

While oxidative stress was comparable, levels of circulating Reclaimers free DNA (cfDNA), and splenic/hepatic and pulmonary translocation of bacteria were decreased and increased, respectively at 48 h.DNases were increased compared to the established models.Proteomic analysis revealed an upregulation of systemic Epo, IL-1b, Prdx5, Parp1, Ccl2 and IL-6 at 48 h in comparison to the healthy controls.Discussion and Conclusion: Midgut volvulus is a stable and physiological model for sepsis.

Depending on the duration and subsequent tissue damage, it represents a combination of ischemia-reperfusion injury and hyperinflammation.